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KMID : 0545119910010010063
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Journal of Microbiology and Biotechnology
1991 Volume.1 No. 1 p.63 ~ p.69
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Performance of Column Type Bioreactor Packed with Immobilized Cyclodextrin Glucanotransferase for Cyclodextrin Production
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Lee, Yong Hyun
Lee, Sang Ho/Shin, Hyun Dong
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Abstract
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Performance of column type bioreactor packed with immobilized cyclodextrin glucanotransferase (CGTase) on chitosan and Amberite IRA 900 was evaluated for cyclodextrin(CD) production. For CGTase immobilized on chitosan, the maximum CD conversion yield of 42% was achieved at the range of 88-168 units of immobilizied CGTase per gram of chitosan, retention time of 0.3 hr, and from 5.0% (w/v) of partially cyclized soluble starch. On the other hand, for CGTase immobilized on Amberite IRA 900, the maximum conversion yield of 40% was obtained at the range of 3.6-11.0 units of immobilized CGTase per gram of carrier and retention time of 1.2 hr from 5.0% of substrate. Above CD conversion yields are almost identical level with that can be obtained with soluble CGTase of 47%. The productivities of bioreactor packed with immobilized CGTase were 17.0g of CD/ℓ·hr for amberite IRA 900 and 15.5 g of CD/ℓ·hr for chitosan. The partially cyclized starch with soluble CGTase were more suitable as substrate to achieve better CD conversion yield, and 5% (w/v) of partially cyclized soluble starch containing 10% (w/w) of CD was found to be most suitable to obtain maximum CD conversion yield.
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